Flexible control

At Lightcast we are developing a platform that combines the flexibility to analyse a wide range of cell types and assays with precise control over droplet number, occupancy, location and movement. Together this enables efficient, multi-step workflows that scale to match your experimental needs.

Technology

Redefine your workflow

Lightcast provides a flexible platform for precisely interrogating single-cell interactions and functional behaviours at scale.

See all operations

Load

Droplet generation & loading

4 specific inputs
Rapidly generate millions of droplets
Optimised for fragile samples

Filter

Droplet acquisition & filtering

Acquisition by light sprites provides precise experimental control
Filter droplets for size and content
Filter criteria automatically defines array formation strategy

Array

Droplet arraying

Machine-learning-optimised array
“Target” droplets contstruct the array while “Disqualified” droplets are discarded
Array is environmentally controlled

Merge

Sample merging

Merge samples by FOV or across the entire chip
Massively parallel merging of interdigitated sample droplets
Conduct complex experiments with multiple sequential merges

Assay

Sample assaying

Define brightfield and fluorescence imaging according to your experimental needs
Identify and select hits with a broad range of functional reporters
Process in real time for down-selection, sequential processing, or dispensing

Identify antigen-binding antibody secretor cells using dynamic single cell analysis

Dispense

Sample dispensing

Dispense selected hits into 96-well plates in real time
Single or pooled cell dispensing based on experimental design
Trace each droplet digitally, from load to dispense.

Precise interrogation of single cell interactions and functional behaviours at scale